Author: Lau, Yee-Ling; Lai, Meng-Yee; Teoh, Boon-Teong; Abd-Jamil, Juraina; Johari, Jefree; Sam, Sing-Sin; Tan, Kim-Kee; AbuBakar, Sazaly
Title: Colorimetric Detection of Dengue by Single Tube Reverse-Transcription-Loop-Mediated Isothermal Amplification Document date: 2015_9_18
ID: 1946zslt_16
Snippet: HNB dye (Sigma, USA) was dissolved in distilled water at 20 mM to prepare a stock solution. The LAMP assay containing 120 μM of HNB dye was performed in a 25 μl reaction mixture with the same components as in the RT-LAMP assay except excluding the (FD) reagent. Turbidity of the RT-LAMP assay was observed with the naked eye. A positive reaction was indicated by a color change from violet to sky blue. On top of that, PCR was performed on 4 μL LA.....
Document: HNB dye (Sigma, USA) was dissolved in distilled water at 20 mM to prepare a stock solution. The LAMP assay containing 120 μM of HNB dye was performed in a 25 μl reaction mixture with the same components as in the RT-LAMP assay except excluding the (FD) reagent. Turbidity of the RT-LAMP assay was observed with the naked eye. A positive reaction was indicated by a color change from violet to sky blue. On top of that, PCR was performed on 4 μL LAMP products using F3 and B3 primers with the same reaction mixture as above with the following PCR cycles: 94°C for 4 min; 35 cycles at 94°C for 30 s, annealing at 55°C for 1 min, extension at 72°C for 30 s.
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