Critical Role of Perforin-dependent CD8+ T Cell Immunity for Rapid Protective Vaccination in a Murine Model for Human Smallpox

Kremer, Melanie; Suezer, Yasemin; Volz, Asisa; Frenz, Theresa; Majzoub, Monir; Hanschmann, Kay-Martin; Lehmann, Michael H.; Kalinke, Ulrich; Sutter, Gerd

Female C57BL/6N mice (6-10 weeks old) were purchased from Charles River Laboratories (Sulzfeld, Germany). C57BL/6J-Igh-6 tm1Cgn mice (mMT, immunoglobulin heavy chain 6 deficient [heavy chain of IgM]) [54] and C57BL/6-Prf1 tm1Sdz /J mice (Prf 2/2 , perforin deficient) [81] . were purchased from The Jackson Laboratory. C57BL/6J-Rag1 tm1Mom mice (RAG-1 2/2 , mice homozygous for the Rag1 tm1Mom mutation produce no mature T cells or B cells) [86] , C57BL/6 MyD88 2/2 TRIF 2/2 mice [47, 87] , C57BL/6 IPS-1 2/2 mice [88] and C57BL/6-Igh-Jtm1Cgn/J mice (JHT, mice homozygous for the Igh-J tm1Cgn targeted mutation fail to produce functional B cells) [55, 89] were bred under specific pathogen-free conditions at the central animal facility of the Paul-Ehrlich Institute. For experimental work, mice were housed in an ISOcage unit (Tecniplast, Germany) and had free access to food and water. All animal experiments were handled in compliance with the German regulations for animal experimentation (Animal Welfare Act).