Author: Cong, Yingying; Kriegenburg, Franziska; de Haan, Cornelis A. M.; Reggiori, Fulvio
Title: Coronavirus nucleocapsid proteins assemble constitutively in high molecular oligomers Document date: 2017_7_18
ID: 15hzah62_5
Snippet: To determine whether the N-protein form homo-dimers or homo-oligomers, we sedimented recombinant 6xHis-tagged N protein on a continuous 5-20% glycerol gradient For comparison sedimentation of the protein standards ovalbumin (44 kDa) and thyroglobulin (669 kDa) was analysed, which could be recovered in fractions 1-2 and 6-8, respectively ( Fig. 1c and d) . GAPDH, which is present in the cytoplasm as a monomer 41 , was detected in the first fractio.....
Document: To determine whether the N-protein form homo-dimers or homo-oligomers, we sedimented recombinant 6xHis-tagged N protein on a continuous 5-20% glycerol gradient For comparison sedimentation of the protein standards ovalbumin (44 kDa) and thyroglobulin (669 kDa) was analysed, which could be recovered in fractions 1-2 and 6-8, respectively ( Fig. 1c and d) . GAPDH, which is present in the cytoplasm as a monomer 41 , was detected in the first fractions of a sedimentation performed with a LR7 cell extract. In contrast, the 6xHis-tagged N protein was exclusively found in fractions 8-11, indicating that it forms large homo-oligomers ( Fig. 1c and d) .
Search related documents:
Co phrase search for related documents- Try single phrases listed below for: 1
Co phrase search for related documents, hyperlinks ordered by date