Author: Delvecchio, Rodrigo; Higa, Luiza M.; Pezzuto, Paula; Valadão, Ana Luiza; Garcez, Patrícia P.; Monteiro, Fábio L.; Loiola, Erick C.; Dias, André A.; Silva, Fábio J. M.; Aliota, Matthew T.; Caine, Elizabeth A.; Osorio, Jorge E.; Bellio, Maria; O’Connor, David H.; Rehen, Stevens; de Aguiar, Renato Santana; Savarino, Andrea; Campanati, Loraine; Tanuri, Amilcar
Title: Chloroquine, an Endocytosis Blocking Agent, Inhibits Zika Virus Infection in Different Cell Models Document date: 2016_11_29
ID: 01s21vh0_9
Snippet: Virus titers were determined by plaque assay performed on Vero cells. Virus stocks or samples were serially diluted and adsorbed to confluent monolayers. After 1 h, the inoculum was removed and cells were overlaid with semisolid medium constituted of alpha-MEM (GIBCO) containing 1% carboxymethyl cellulose (Sigma Aldrich) and 1% FBS (GIBCO). Cells were further incubated for 5 days when cells were fixed in 4% formaldehyde. Cells were stained with 1.....
Document: Virus titers were determined by plaque assay performed on Vero cells. Virus stocks or samples were serially diluted and adsorbed to confluent monolayers. After 1 h, the inoculum was removed and cells were overlaid with semisolid medium constituted of alpha-MEM (GIBCO) containing 1% carboxymethyl cellulose (Sigma Aldrich) and 1% FBS (GIBCO). Cells were further incubated for 5 days when cells were fixed in 4% formaldehyde. Cells were stained with 1% crystal violet in 20% ethanol for plaque visualization. Titers were expressed as plaque forming units (PFU) per milliliter.
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