Author: Cong, Yingying; Kriegenburg, Franziska; de Haan, Cornelis A. M.; Reggiori, Fulvio
Title: Coronavirus nucleocapsid proteins assemble constitutively in high molecular oligomers Document date: 2017_7_18
ID: 15hzah62_19
Snippet: Our attempts to narrow down the specific binding area within the various regions have been unsuccessful since it appears that the amino acids crucial for the N protein self-interaction are discontinuously distributed (Fig. 3) . The results obtained with the N2a truncation indirectly support this notion as a short domain will possess less key binding amino acids and consequently it will interact less pronouncedly compared to longer parts such as t.....
Document: Our attempts to narrow down the specific binding area within the various regions have been unsuccessful since it appears that the amino acids crucial for the N protein self-interaction are discontinuously distributed (Fig. 3) . The results obtained with the N2a truncation indirectly support this notion as a short domain will possess less key binding amino acids and consequently it will interact less pronouncedly compared to longer parts such as the N1 and N2b-N3 domains. Previous efforts to localize the residues essential to mediate SARS-CoV N2b-N3 (including the CTD) multimerization identified three different regions within this domain 22, 31, 34 , further underscoring the notion that discontinues binding regions, distributed over the entire N molecule, are responsible for the self-association. During the preparation of this manuscript, a cryo-EM analysis of ribonucleoprotein complexes isolated MHV virions was published and the model emerging from this work is consistent with our findings 48 . This study suggested that the N protein form octamers mainly via its CTD domain, which then further assembles into larger oligomeric structures that can acquire either a loose or a more compact intertwined filament shape 48 . In the proposed model, multiple surfaces of the N protein participate in the multimerization of the N protein octamer and this is coherent with our conclusion that several domains in the N protein mediate self-interaction.
Search related documents:
Co phrase search for related documents- amino acid and bind region: 1, 2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12
- amino acid and crucial amino acid: 1, 2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17, 18, 19, 20, 21, 22
- amino acid and cryo em: 1, 2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17, 18, 19, 20, 21, 22, 23, 24, 25
- amino acid and cryo em analysis: 1, 2
- amino acid and CTD domain: 1, 2, 3, 4, 5, 6, 7, 8, 9
- amino acid and different region: 1, 2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14
- amino acid and essential residue: 1, 2, 3, 4, 5, 6, 7
- bind region and different region: 1, 2
- cryo em and CTD domain: 1, 2, 3
- cryo em and different region: 1
- different region and essential residue: 1
Co phrase search for related documents, hyperlinks ordered by date