Selected article for: "ELISA result and LAMP assay"
Author: Lau, Yee-Ling; Lai, Meng-Yee; Teoh, Boon-Teong; Abd-Jamil, Juraina; Johari, Jefree; Sam, Sing-Sin; Tan, Kim-Kee; AbuBakar, Sazaly
Title: Colorimetric Detection of Dengue by Single Tube Reverse-Transcription-Loop-Mediated Isothermal Amplification
  • Document date: 2015_9_18
    • ID: 1946zslt_24
    Snippet: The clinical sensitivity and specificity of RT-LAMP assay was calculated based on the screening of the 189 suspected dengue and 24 healthy donor serum samples. Sensitivity was calculated as (number of true positives)/(number of true positives + number of false negatives), and specificity was calculated as (number of true negatives)/(number of true negatives + number of false positives). Positive predictive value (PPV) and Negative predictive valu.....
    Document: The clinical sensitivity and specificity of RT-LAMP assay was calculated based on the screening of the 189 suspected dengue and 24 healthy donor serum samples. Sensitivity was calculated as (number of true positives)/(number of true positives + number of false negatives), and specificity was calculated as (number of true negatives)/(number of true negatives + number of false positives). Positive predictive value (PPV) and Negative predictive value (NPV) were calculated based on an average of 84% of dengue prevalence in Malaysia [12] . PPV = (sensitivity X prevalence)/ [sensitivity X prevalence + (1-specificity) X (1-prevalence)], NPV = [specificity X (1-prevalence)]/ [(1-sensitivity) X prevalence + specificity X (1-prevalence)]. A composite diagnosis for each sample (2 out of 3 tests based on qRT-PCR, ELISA and RT-LAMP giving the same result) was used as a reference.

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