Selected article for: "QIAGEN RNeasy minikit and RNeasy minikit"
Author: Brandão, Paulo E.; Hora, Aline S.; Silva, Sheila O. S.; Berg, Mikael; Taniwaki, Sueli A.
Title: Complete Genome Sequence of Avian Coronavirus Strain D274
  • Document date: 2018_8_30
    • ID: 0z0uabfw_3
    Snippet: The Gammacoronavirus/AvCoV/chicken/Netherlands/D274/1979 IBV strain (lineage GI-12) was isolated from tissue samples of chickens collected in 1979 in The Netherlands (5) and is used as an inactivated intramuscular vaccine strain in several countries, including Brazil. An aliquot of a D274 strain used in an inactivated vaccine was clarified at 12,000 ϫ g/15 min at 4°C and then filtered (0.45 m) and treated with DNase/RNase, and the next total RN.....
    Document: The Gammacoronavirus/AvCoV/chicken/Netherlands/D274/1979 IBV strain (lineage GI-12) was isolated from tissue samples of chickens collected in 1979 in The Netherlands (5) and is used as an inactivated intramuscular vaccine strain in several countries, including Brazil. An aliquot of a D274 strain used in an inactivated vaccine was clarified at 12,000 ϫ g/15 min at 4°C and then filtered (0.45 m) and treated with DNase/RNase, and the next total RNA was purified using TRIzol reagent (Life Technologies) and an RNeasy minikit (Qiagen). SuperScript III and Klenow exo-DNA polymerase (Life Technologies) were used to produce double-stranded cDNAs (ds-cDNAs) using total RNA containing 5,000 copies/l of IBV genome as measured with a quantitative PCR (qPCR) modified from reference 6.

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