Current Approaches on Viral Infection: Proteomics and Functional Validations

Zheng, Jie; Tan, Boon Huan; Sugrue, Richard; Tang, Kai

Tandem affinity purification based MS approach Protein-protein interactions Jorba et al. (2008) , Mayer et al. (2005) , Mayer et al. (2007) Co-immunoprecipitation based MS approach Binding partners of target protein Moresco et al. (2010) , Noisakran et al. (2008) HLA peptidome scanning chip based MS approach Searching for disease related peptides or differently expressed proteins after virus infection Herberts et al. (2003) , Wahl et al. (2010) Isotope coded affinity tag (ICAT) Mapping differently expressed proteins in host proteome upon virus infection Yan et al. (2004) , Booy et al. (2005) Isobaric tag for relative and absolute quantitation (iTRAQ) Zhang et al. (2009) , Chen et al. (2008) Stable isotope labeling of amino acids in cell culture (SILAC) Dreger et al. (2009) , Hammond et al. (2010) Difference gel electrophoresis (DIGE) Pastorino et al. (2009) , Sun et al. (2011a) Cross-linking based MS approach Investigating protein-nucleotides binding sites Deval et al. (2007) , Bhardwaj et al. (2008) Hydrogen/deuterium exchange (HDX) based MS approach etc. Conformational dynamics of host or viral proteins Lisal et al. (2005) , Monroe et al. (2010) bridges. These cross-links could be reversed by heating and the eluted peptides are further submitted to MS for sequence identifications (Orlando et al., 1997; Metz et al., 2004) . For its applications, the helicase-like regions within the viral polymerase involved in RNA binding were characterized by reversible formaldehyde crosslinking and MS (Kim et al., 2005; Deval et al., 2007; Han et al., 2009) .