Selected article for: "density map and electron density map"
Author: Xu, Xiaoling; Lou, Zhiyong; Ma, Yanlin; Chen, Xuehui; Yang, Zhangsheng; Tong, Xiaohang; Zhao, Qi; Xu, Yuanyuan; Deng, Hongyu; Bartlam, Mark; Rao, Zihe
Title: Crystal Structure of the C-Terminal Cytoplasmic Domain of Non-Structural Protein 4 from Mouse Hepatitis Virus A59
  • Document date: 2009_7_10
    • ID: 1beonuh7_36
    Snippet: The structure of nsp4C was solved by the single-wavelength anomalous dispersion (SAD) method from a Se-Met derivative crystal. Three out of the four expected selenium atoms in one molecule were located and initial phases were calculated by the program SOLVE [43] . After density modification (solvent flipping) using the program RESOLVE, a clearly interpretable electron density map was calculated. The model of nsp4C was then manually built and refi.....
    Document: The structure of nsp4C was solved by the single-wavelength anomalous dispersion (SAD) method from a Se-Met derivative crystal. Three out of the four expected selenium atoms in one molecule were located and initial phases were calculated by the program SOLVE [43] . After density modification (solvent flipping) using the program RESOLVE, a clearly interpretable electron density map was calculated. The model of nsp4C was then manually built and refined using the programs O [44] and CNS [45] . The crystal structure was refined at 2.0 Ã… resolution to a final R work of 22.1% and R free of 26.4%. The structure of the C425S mutant was solved by molecular replacement (MR). A model was obtained with the program PHASER from the CCP4 package [46], then further refined and rebuilt with CNS and O respectively. The crystal structure of the C425S mutant was refined at 2.4 Ã… resolution to a final R work of 22.7% and R free of 26.6%. Refinement statistics are detailed in Table 1 .

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