Author: Shields, Lauren E.; Jennings, Jordan; Liu, Qinfang; Lee, Jinhwa; Ma, Wenjun; Blecha, Frank; Miller, Laura C.; Sang, Yongming
Title: Cross-Species Genome-Wide Analysis Reveals Molecular and Functional Diversity of the Unconventional Interferon-? Subtype Document date: 2019_6_25
ID: 14gcu1se_24
Snippet: Cellular viability was assessed by trypan blue dye exclusion (Invitrogen, Carlsbad, CA). Five-hundred cells in 100 µL volume were added to each well of a flat-bottom 96-well plate in triplicate. Cells were treated with or without IFN peptides at the indicated concentrations (1:10 serially diluted from 2 ng/ml) and incubated at 37 • C for 72 h. Cell growth was determined by a MTS-salt [3-(4,5-dimethythiazol-2-yl)]-5-(3carboxymethoxyphenyl)-2-(4.....
Document: Cellular viability was assessed by trypan blue dye exclusion (Invitrogen, Carlsbad, CA). Five-hundred cells in 100 µL volume were added to each well of a flat-bottom 96-well plate in triplicate. Cells were treated with or without IFN peptides at the indicated concentrations (1:10 serially diluted from 2 ng/ml) and incubated at 37 • C for 72 h. Cell growth was determined by a MTS-salt [3-(4,5-dimethythiazol-2-yl)]-5-(3carboxymethoxyphenyl)-2-(4-sulfophenyl)-2H-tetrazolium) assay (Promega, Madison, WI). Briefly, 20 µl of MTS reagent were added to each well and the plate incubated for 2-4 h at 37 • C. Following color development, absorbance was measured at 490 nm on a SpectraMax i3 (Molecular Devices) spectrometer (23, 26) .
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