Author: Selinger, Christian; Tisoncik-Go, Jennifer; Menachery, Vineet D; Agnihothram, Sudhakar; Law, G Lynn; Chang, Jean; Kelly, Sara M; Sova, Pavel; Baric, Ralph S; Katze, Michael G
Title: Cytokine systems approach demonstrates differences in innate and pro-inflammatory host responses between genetically distinct MERS-CoV isolates Document date: 2014_12_22
ID: 0y3m47lh_20
Snippet: Calu-3 2B4 cells were cultured in minimal essential media (MEM; Gibco) supplemented with 20% fetal bovine serum (HyClone) and 1% antibiotic antimycotic (Gibco [2] , where isolation of viruses from clinical samples is described. Virus stocks were tittered on VeroE6 cells using standard methods, as previously described [14] . SARS-CoV experiments were derived from the infectious clone of SARS-CoV (icSARS-CoV) as described in [18, 38] ......
Document: Calu-3 2B4 cells were cultured in minimal essential media (MEM; Gibco) supplemented with 20% fetal bovine serum (HyClone) and 1% antibiotic antimycotic (Gibco [2] , where isolation of viruses from clinical samples is described. Virus stocks were tittered on VeroE6 cells using standard methods, as previously described [14] . SARS-CoV experiments were derived from the infectious clone of SARS-CoV (icSARS-CoV) as described in [18, 38] .
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