Author: Van Nguyen, Dung; Van Nguyen, Cuong; Bonsall, David; Ngo, Tue Tri; Carrique-Mas, Juan; Pham, Anh Hong; Bryant, Juliet E.; Thwaites, Guy; Baker, Stephen; Woolhouse, Mark; Simmonds, Peter
Title: Detection and Characterization of Homologues of Human Hepatitis Viruses and Pegiviruses in Rodents and Bats in Vietnam Document date: 2018_2_28
ID: 0jtfc271_11
Snippet: Liver samples from 157 bats and 470 rodents were subjected to nucleic acid extraction using AllPrep DNA/RNA Mini Kit (Qiagen, Manchester, UK). Briefly, about 30 mg of liver per sample was first lysed and homogenised using TissueLyser (Qiagen, Manchester, UK). The lysate was applied to an AllPrep DNA spin column for DNA to bind onto the column. Ethanol was added to the flow-through and RNA and bound to the membrane when the sample was passed throu.....
Document: Liver samples from 157 bats and 470 rodents were subjected to nucleic acid extraction using AllPrep DNA/RNA Mini Kit (Qiagen, Manchester, UK). Briefly, about 30 mg of liver per sample was first lysed and homogenised using TissueLyser (Qiagen, Manchester, UK). The lysate was applied to an AllPrep DNA spin column for DNA to bind onto the column. Ethanol was added to the flow-through and RNA and bound to the membrane when the sample was passed through an RNeasy spin column. After washing steps, DNA and RNA was eluted separately in 50 µL of nuclease-free water. Extracted nucleic acid was used in screening for the targeted hepatitis viruses.
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