Selected article for: "rna bind and RNase treatment"
Author: Cong, Yingying; Kriegenburg, Franziska; de Haan, Cornelis A. M.; Reggiori, Fulvio
Title: Coronavirus nucleocapsid proteins assemble constitutively in high molecular oligomers
  • Document date: 2017_7_18
    • ID: 15hzah62_4
    Snippet: Since it has been shown that CoV N proteins also bind non-viral RNA 19, 21, [37] [38] [39] [40] , we also investigated the association between purified GST-tagged and 6xHis-tagged N proteins after RNase A treatment to exclude a role of bacterial RNA in the assayed interaction. As shown in Supplementary Fig. S1a , RNase A treatment removed large part of the RNA present in the bacterial extract and nucleic acids were not detected associated to the .....
    Document: Since it has been shown that CoV N proteins also bind non-viral RNA 19, 21, [37] [38] [39] [40] , we also investigated the association between purified GST-tagged and 6xHis-tagged N proteins after RNase A treatment to exclude a role of bacterial RNA in the assayed interaction. As shown in Supplementary Fig. S1a , RNase A treatment removed large part of the RNA present in the bacterial extract and nucleic acids were not detected associated to the purified N protein even when this was not exposed to RNase A. Importantly, removal of RNA did not affect the binding between the two N protein fusions ( Supplementary Fig. S1b ). We concluded that the bacterial RNA does not participate in the in vitro interaction of MHV N protein.

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