Author: Turgeon, Nathalie; Hamelin, Marie-Ève; Verreault, Daniel; Lévesque, Ariane; Rhéaume, Chantal; Carbonneau, Julie; Checkmahomed, Liva; Girard, Matthieu; Boivin, Guy; Duchaine, Caroline
Title: Design and Validation with Influenza A Virus of an Aerosol Transmission Chamber for Ferrets Document date: 2019_2_19
ID: 1pcy24po_14
Snippet: Three groups of three seronegative (800-to 1000-g) male ferrets (Triple F Farms Inc., Gillett, PA, USA) were housed consecutively in the system for 7 to 12 days. The ventilation system was set at 200 L/min, with 160 holes of the particle separator for all experiments. Ferrets housed in cage one were infected intra-nasally with 250 µL (125 µL per nostril) containing 4.5 log TCID50/mL of the A/California/7/2009 (H1N1) influenza A virus. Nasal was.....
Document: Three groups of three seronegative (800-to 1000-g) male ferrets (Triple F Farms Inc., Gillett, PA, USA) were housed consecutively in the system for 7 to 12 days. The ventilation system was set at 200 L/min, with 160 holes of the particle separator for all experiments. Ferrets housed in cage one were infected intra-nasally with 250 µL (125 µL per nostril) containing 4.5 log TCID50/mL of the A/California/7/2009 (H1N1) influenza A virus. Nasal wash was collected every day by instillation of 5 mL Phosphate-Buffered Saline (PBS) into the intranasal cavity.
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