Author: Yu, Fei; Qiu, Ting; Zeng, Ying; Wang, Yiyin; Zheng, Shufa; Chen, Xiao; Chen, Yu
Title: Comparative Evaluation of Three Preprocessing Methods for Extraction and Detection of Influenza A Virus Nucleic Acids from Sputum Document date: 2018_3_2
ID: 0kt9b1ww_5
Snippet: Proteinase K (PK), a common component in the nucleic acid isolation kit of blood specimens, is a serine protease that exhibits broad cleavage specificity (4) and degrades RNases in samples, resulting in prevention of RNA degradation (3) . The sulfhydryl reagent dithiothreitol (DTT), a superior reagent to reduce mucoprotein disulfide bonds specifically and completely, is widely used for sputum sample homogenization (5) (6) (7) . Therefore, in this.....
Document: Proteinase K (PK), a common component in the nucleic acid isolation kit of blood specimens, is a serine protease that exhibits broad cleavage specificity (4) and degrades RNases in samples, resulting in prevention of RNA degradation (3) . The sulfhydryl reagent dithiothreitol (DTT), a superior reagent to reduce mucoprotein disulfide bonds specifically and completely, is widely used for sputum sample homogenization (5) (6) (7) . Therefore, in this study, we compared these three preprocessing methods of sputum specimens on the extraction and detection of IAV nucleic acids to provide a basis for the laboratory preprocessing of sputum specimens.
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