Selected article for: "isolation kit and nucleic acid"
Author: Yu, Fei; Qiu, Ting; Zeng, Ying; Wang, Yiyin; Zheng, Shufa; Chen, Xiao; Chen, Yu
Title: Comparative Evaluation of Three Preprocessing Methods for Extraction and Detection of Influenza A Virus Nucleic Acids from Sputum
  • Document date: 2018_3_2
    • ID: 0kt9b1ww_5
    Snippet: Proteinase K (PK), a common component in the nucleic acid isolation kit of blood specimens, is a serine protease that exhibits broad cleavage specificity (4) and degrades RNases in samples, resulting in prevention of RNA degradation (3) . The sulfhydryl reagent dithiothreitol (DTT), a superior reagent to reduce mucoprotein disulfide bonds specifically and completely, is widely used for sputum sample homogenization (5) (6) (7) . Therefore, in this.....
    Document: Proteinase K (PK), a common component in the nucleic acid isolation kit of blood specimens, is a serine protease that exhibits broad cleavage specificity (4) and degrades RNases in samples, resulting in prevention of RNA degradation (3) . The sulfhydryl reagent dithiothreitol (DTT), a superior reagent to reduce mucoprotein disulfide bonds specifically and completely, is widely used for sputum sample homogenization (5) (6) (7) . Therefore, in this study, we compared these three preprocessing methods of sputum specimens on the extraction and detection of IAV nucleic acids to provide a basis for the laboratory preprocessing of sputum specimens.

    Search related documents:
    Co phrase search for related documents
    • broad cleavage specificity and cleavage specificity: 1
    • cleavage specificity and RNA degradation: 1
    • cleavage specificity and rnase degrade: 1
    • cleavage specificity and serine protease: 1, 2
    • detection extraction and IAV nucleic acid: 1, 2, 3
    • detection extraction and IAV nucleic acid detection extraction: 1
    • detection extraction and isolation kit: 1
    • detection extraction and nucleic acid: 1, 2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17, 18, 19, 20, 21, 22, 23, 24, 25, 26, 27, 28, 29, 30, 31, 32, 33, 34, 35, 36, 37, 38, 39, 40, 41, 42, 43, 44, 45, 46, 47, 48, 49, 50, 51, 52, 53, 54, 55, 56, 57, 58, 59, 60, 61, 62, 63, 64, 65, 66, 67, 68, 69
    • detection extraction and preprocessing method: 1
    • disulfide bond and nucleic acid: 1, 2
    • IAV nucleic acid and nucleic acid: 1, 2, 3, 4