Selected article for: "fragment precursor and mass tolerance"
Author: Chernobrovkin, Alexey L.; Zubarev, Roman A.
Title: Detection of Viral Proteins in Human Cells Lines by Xeno-Proteomics: Elimination of the Last Valid Excuse for Not Testing Every Cellular Proteome Dataset for Viral Proteins
  • Document date: 2014_3_11
    • ID: 0hrwqho8_16
    Snippet: Trypsin specificity was used in MS/MS search with up to two missed cleavages. N-terminal acetylation and methionine oxidation were selected as variable modifications, while carbamidomethylation of cysteine was selected as a fixed modification. Precursor ion mass accuracy was set to 15 ppm, fragment ion mass tolerance to 30 mDa. Automatic decoy database search and builtin Percolator algorithm (version 2.01) [31] were used to rescore search results.....
    Document: Trypsin specificity was used in MS/MS search with up to two missed cleavages. N-terminal acetylation and methionine oxidation were selected as variable modifications, while carbamidomethylation of cysteine was selected as a fixed modification. Precursor ion mass accuracy was set to 15 ppm, fragment ion mass tolerance to 30 mDa. Automatic decoy database search and builtin Percolator algorithm (version 2.01) [31] were used to rescore search results and calculate posterior error probabilities for each peptide-spectrum match. All results were filtered to achieve global FDR #1%.

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