Author: Moore, Matthew D.; Jaykus, Lee-Ann
Title: Development of a Recombinase Polymerase Amplification Assay for Detection of Epidemic Human Noroviruses Document date: 2017_1_9
ID: 03a6hq3t_20
Snippet: Real time RT-RPA primer and probe design. Primers for RT-RPA were designed following manufacturer guidelines (TwistDx Inc. Cambridge, UK). Targets for primer design were the relatively conserved ORF1-2 junction and a previously reported conserved region of the norovirus capsid near the N/S domain of ORF2 15, 38 . After initial screening, one optimally performing primer set was selected and the corresponding probe was designed according to manufac.....
Document: Real time RT-RPA primer and probe design. Primers for RT-RPA were designed following manufacturer guidelines (TwistDx Inc. Cambridge, UK). Targets for primer design were the relatively conserved ORF1-2 junction and a previously reported conserved region of the norovirus capsid near the N/S domain of ORF2 15, 38 . After initial screening, one optimally performing primer set was selected and the corresponding probe was designed according to manufacturer's instructions (TwistDx Inc.) ( Table 1 ). Primers were provided by Integrated DNA Technologies (IDT, Coralville, IA) and probes were provided by Biosearch Technologies (Novato, CA).
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