Author: Turgeon, Nathalie; Hamelin, Marie-Ève; Verreault, Daniel; Lévesque, Ariane; Rhéaume, Chantal; Carbonneau, Julie; Checkmahomed, Liva; Girard, Matthieu; Boivin, Guy; Duchaine, Caroline
Title: Design and Validation with Influenza A Virus of an Aerosol Transmission Chamber for Ferrets Document date: 2019_2_19
ID: 1pcy24po_12
Snippet: The polydispersed nanometer particle size was generated using a collision 6-jet nebulizer (BGI, Waltham, MA, USA), filled with 50 mL of buffer (20 mM Tris-HCl, 100 mM NaCl, 10 mM MgSO4, pH 7.5) and supplied with 20 psi. Aerosols were passing through a diffusion dryer (model 3062, TSI Inc. Shoreview, MN, USA) and a neutralizer (model 3012A, TSI Inc. Shoreview, MN, USA) before entering the chamber in cage number one. Nebulization was started 30 min.....
Document: The polydispersed nanometer particle size was generated using a collision 6-jet nebulizer (BGI, Waltham, MA, USA), filled with 50 mL of buffer (20 mM Tris-HCl, 100 mM NaCl, 10 mM MgSO4, pH 7.5) and supplied with 20 psi. Aerosols were passing through a diffusion dryer (model 3062, TSI Inc. Shoreview, MN, USA) and a neutralizer (model 3012A, TSI Inc. Shoreview, MN, USA) before entering the chamber in cage number one. Nebulization was started 30 min before starting measurements to stabilize the particle concentration in the three chambers. Measurements were performed using a NanoScan SMPS (model 3910, TSI Inc. Shoreview, MN, USA). The NanoScan sampled cage two for 10 min and then sampled cage three for 10 min. The experiments were repeated three times in all conditions. Particle size distribution as well as total nanoparticles were compared between cages two and three.
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