Author: Chernobrovkin, Alexey L.; Zubarev, Roman A.
Title: Detection of Viral Proteins in Human Cells Lines by Xeno-Proteomics: Elimination of the Last Valid Excuse for Not Testing Every Cellular Proteome Dataset for Viral Proteins Document date: 2014_3_11
ID: 0hrwqho8_25
Snippet: Both envelope proteins and gag and pol DNA sequences observed by PCR in EKVX cell line, have not been detected in any other NCI60 cell line [28] . We used the proteomics data on the remaining 58 NCI-60 cell datasets [29] as a negative control for our procedure. The data quality (resolution in MS and MS/ MS) for nine cell lines MCF7, M14, COLO205, CCRFCEM, U251, H460, PC3, SKOV3 and RXF393) was similar to that in Geiger et al. [18] , whereas 49 ce.....
Document: Both envelope proteins and gag and pol DNA sequences observed by PCR in EKVX cell line, have not been detected in any other NCI60 cell line [28] . We used the proteomics data on the remaining 58 NCI-60 cell datasets [29] as a negative control for our procedure. The data quality (resolution in MS and MS/ MS) for nine cell lines MCF7, M14, COLO205, CCRFCEM, U251, H460, PC3, SKOV3 and RXF393) was similar to that in Geiger et al. [18] , whereas 49 cell lines were represented by lowresolution CID MS/MS spectra. We were able to observe in highresolution MS/MS data from 6000 to 8000 human protein groups and 2800-4000 groups in low resolution MS/MS data. Not a single viral peptide was detected with #1% FDR in either of the 58 cell lines.
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