Selected article for: "detection limit and human norovirus detection"
Author: Moore, Matthew D.; Jaykus, Lee-Ann
Title: Development of a Recombinase Polymerase Amplification Assay for Detection of Epidemic Human Noroviruses
  • Document date: 2017_1_9
    • ID: 03a6hq3t_11
    Snippet: For point-of-care diagnostics in particular, isothermal amplification methods are of great interest due to their convenience, rapid time-to-result, and potential for miniaturization. Two of these methods have been well studied for human norovirus detection: nucleic acid sequence based amplification (NASBA) and loop-mediated isothermal amplification (LAMP). Greene et al. 12 developed the first norovirus NASBA, targeting Norwalk virus, reporting a .....
    Document: For point-of-care diagnostics in particular, isothermal amplification methods are of great interest due to their convenience, rapid time-to-result, and potential for miniaturization. Two of these methods have been well studied for human norovirus detection: nucleic acid sequence based amplification (NASBA) and loop-mediated isothermal amplification (LAMP). Greene et al. 12 developed the first norovirus NASBA, targeting Norwalk virus, reporting a detection limit of 10 4 PCR-amplifiable units/ml sample and a total time-to-result of 4-6 h. Moore et al. 13 evaluated the sensitivity and specificity of this same NASBA using GI, GII, and outbreak strains. The method 34 3.0-9.0 7.4 ± 0.5-13.4 ± 2.6 5.0 (3/3) 3.6-9.6 7.8 ± 1.5-11.8 ± 0.0 c 6.6 (1/3)

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