Selected article for: "high specificity and positive signal"
Author: Moore, Matthew D.; Jaykus, Lee-Ann
Title: Development of a Recombinase Polymerase Amplification Assay for Detection of Epidemic Human Noroviruses
  • Document date: 2017_1_9
    • ID: 03a6hq3t_15
    Snippet: Scientific RepoRts | 7:40244 | DOI: 10.1038/srep40244 a high degree of secondary structure; and (v.) a reduced time to a positive signal. A disadvantage with respect to human norovirus detection may be lack of broad reactivity (i.e., high specificity), perhaps because the recombinase enzyme used is thought to have higher fidelity than PCR 6, 7 . However, in our work some degree of base mismatch appears to have been tolerated as we were able to de.....
    Document: Scientific RepoRts | 7:40244 | DOI: 10.1038/srep40244 a high degree of secondary structure; and (v.) a reduced time to a positive signal. A disadvantage with respect to human norovirus detection may be lack of broad reactivity (i.e., high specificity), perhaps because the recombinase enzyme used is thought to have higher fidelity than PCR 6, 7 . However, in our work some degree of base mismatch appears to have been tolerated as we were able to detect two different GII.4 strains, although older strains have not been evaluated. However, having a method that reliably detects GII.4 norovirus is relevant as this genotype is responsible for at least 70% of norovirus cases in the United States 23 and 80-95% of outbreaks globally 24 . New RT-RPA assays could be redesigned relatively quickly to cover new epidemic strains as they emerge.

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