Author: Moore, Matthew D.; Jaykus, Lee-Ann
Title: Development of a Recombinase Polymerase Amplification Assay for Detection of Epidemic Human Noroviruses Document date: 2017_1_9
ID: 03a6hq3t_9
Snippet: In this work, a RT-RPA assay for the rapid detection of GII.4 human norovirus was developed and proof-of-concept data provided for its use in detecting virus in representative clinical samples. In purified RNA extracts, the assay limit of detection was 3.40 LGC per reaction. When human fecal samples positive for norovirus GII.4 New Orleans were extracted for RNA isolation followed by RT-RPA, virus could be detected in all patient specimens, with .....
Document: In this work, a RT-RPA assay for the rapid detection of GII.4 human norovirus was developed and proof-of-concept data provided for its use in detecting virus in representative clinical samples. In purified RNA extracts, the assay limit of detection was 3.40 LGC per reaction. When human fecal samples positive for norovirus GII.4 New Orleans were extracted for RNA isolation followed by RT-RPA, virus could be detected in all patient specimens, with longer amplification times to target signal corresponding to lower input template concentrations. It was possible to detect norovirus when 20% fecal specimens were heated to release the viral RNA, without further purification. With this heat release method, lower limits of detection were around 5.0 LGC per reaction.
Search related documents:
Co phrase search for related documents- assay limit and concept proof: 1, 2, 3, 4, 5
- assay limit and detection assay limit: 1, 2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17, 18, 19, 20, 21, 22, 23, 24, 25
- assay limit and detection low limit: 1, 2, 3, 4, 5, 6, 7, 8
- assay limit and input template: 1
- assay limit and low limit: 1, 2, 3, 4, 5, 6, 7, 8, 9, 10
- assay limit and norovirus detect: 1
- clinical sample and concept proof: 1, 2
- clinical sample and detection assay limit: 1, 2, 3, 4, 5, 6, 7
- clinical sample and detection low limit: 1, 2
- clinical sample and fecal sample: 1, 2, 3, 4, 5
- clinical sample and low limit: 1, 2
- clinical sample and patient specimen: 1
- concept proof and detection assay limit: 1, 2, 3, 4, 5
- concept proof and detection low limit: 1, 2
- concept proof and low limit: 1, 2, 3, 4
- detection assay limit and input template: 1
- detection assay limit and low limit: 1, 2, 3, 4, 5, 6, 7, 8, 9, 10
- detection assay limit and norovirus detect: 1
- detection low limit and low limit: 1, 2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17, 18, 19, 20, 21, 22, 23, 24, 25
Co phrase search for related documents, hyperlinks ordered by date