Selected article for: "MS MS data and MS spectra"

Author: Chernobrovkin, Alexey L.; Zubarev, Roman A.
Title: Detection of Viral Proteins in Human Cells Lines by Xeno-Proteomics: Elimination of the Last Valid Excuse for Not Testing Every Cellular Proteome Dataset for Viral Proteins
  • Document date: 2014_3_11
  • ID: 0hrwqho8_11
    Snippet: The procedure consists of label-free proteomics analysis with subsequent identification of human as well as non-human proteins by the tandem mass spectra (MS/MS) of their peptides. We addressed the fact of unequal a priori probabilities for viral and human peptide matches by identifying all human-related MS/MS spectra first, leaving for subsequent identification only non-human data. The final stage of the procedure comprises quantitative estimati.....
    Document: The procedure consists of label-free proteomics analysis with subsequent identification of human as well as non-human proteins by the tandem mass spectra (MS/MS) of their peptides. We addressed the fact of unequal a priori probabilities for viral and human peptide matches by identifying all human-related MS/MS spectra first, leaving for subsequent identification only non-human data. The final stage of the procedure comprises quantitative estimation of viral protein abundances in comparison to the host proteome, to determine the severity of contamination.

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