Author: Chernobrovkin, Alexey L.; Zubarev, Roman A.
Title: Detection of Viral Proteins in Human Cells Lines by Xeno-Proteomics: Elimination of the Last Valid Excuse for Not Testing Every Cellular Proteome Dataset for Viral Proteins Document date: 2014_3_11
ID: 0hrwqho8_23
Snippet: To test whether the procedure can detect viral contamination that is almost surely present in the sampled cells, we analyzed proteomic data of EKVX cell lines from the NCI60 panel. The presence of viral infection in this cell line has been detected during the screening of the NCI60 panel for envelope proteins and gene sequences related to xenotropic murine leukemia viruses (X-MLVs) [28] . The shotgun proteomic analysis of the EKVX cell line descr.....
Document: To test whether the procedure can detect viral contamination that is almost surely present in the sampled cells, we analyzed proteomic data of EKVX cell lines from the NCI60 panel. The presence of viral infection in this cell line has been detected during the screening of the NCI60 panel for envelope proteins and gene sequences related to xenotropic murine leukemia viruses (X-MLVs) [28] . The shotgun proteomic analysis of the EKVX cell line described elsewhere [29] provided 144,926 MS/MS spectra, 62,450 of which were assigned by Mascot to the 3,613 human proteins. Using the proposed three-step approach, we identified both env (ENV_XMRV3) and pol (POL_XMRV3) viral proteins, which is consistent with the PCR-based results obtained on the same cell line in a different [28] study.
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