Author: Nakamura, Shota; Yang, Cheng-Song; Sakon, Naomi; Ueda, Mayo; Tougan, Takahiro; Yamashita, Akifumi; Goto, Naohisa; Takahashi, Kazuo; Yasunaga, Teruo; Ikuta, Kazuyoshi; Mizutani, Tetsuya; Okamoto, Yoshiko; Tagami, Michihira; Morita, Ryoji; Maeda, Norihiro; Kawai, Jun; Hayashizaki, Yoshihide; Nagai, Yoshiyuki; Horii, Toshihiro; Iida, Tetsuya; Nakaya, Takaaki
Title: Direct Metagenomic Detection of Viral Pathogens in Nasal and Fecal Specimens Using an Unbiased High-Throughput Sequencing Approach Document date: 2009_1_19
ID: 1j8z8lak_6
Snippet: Total RNA isolated from either nasopharyngeal aspirates or fecal samples (0.1-0.2 ml) was under-measurable with an ND-1000 spectrophotometer (NanoDrop Technologies). Therefore, we performed quasi-random RT-PCR amplification using a whole transcriptome amplification (WTA) kit, according to the manufacturer's protocol with modifications, i.e. 70 cycles of PCR [21] . After random RT-PCR amplification, 10-13 mg of cDNA were obtained from the nasal an.....
Document: Total RNA isolated from either nasopharyngeal aspirates or fecal samples (0.1-0.2 ml) was under-measurable with an ND-1000 spectrophotometer (NanoDrop Technologies). Therefore, we performed quasi-random RT-PCR amplification using a whole transcriptome amplification (WTA) kit, according to the manufacturer's protocol with modifications, i.e. 70 cycles of PCR [21] . After random RT-PCR amplification, 10-13 mg of cDNA were obtained from the nasal and fecal samples ( Figure 1A ).
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