Author: Nakamura, Shota; Yang, Cheng-Song; Sakon, Naomi; Ueda, Mayo; Tougan, Takahiro; Yamashita, Akifumi; Goto, Naohisa; Takahashi, Kazuo; Yasunaga, Teruo; Ikuta, Kazuyoshi; Mizutani, Tetsuya; Okamoto, Yoshiko; Tagami, Michihira; Morita, Ryoji; Maeda, Norihiro; Kawai, Jun; Hayashizaki, Yoshihide; Nagai, Yoshiyuki; Horii, Toshihiro; Iida, Tetsuya; Nakaya, Takaaki
Title: Direct Metagenomic Detection of Viral Pathogens in Nasal and Fecal Specimens Using an Unbiased High-Throughput Sequencing Approach Document date: 2009_1_19
ID: 1j8z8lak_33
Snippet: Viral RNA was extracted from nasopharyngeal and fecal specimens with a QIAamp Viral RNA Mini Kit (QIAGEN), and cDNA was synthesized using SuperScript TM III reverse transcriptase (Invitrogen) with a random hexamer, as described previously [21] . The generated cDNA was subjected to PCR using the Expand High Fidelity PLUS PCR System (Roche) with primer sets specific to viruses, such as human coronaviruses [38] , WU polyomavirus [24] , and PMMV [31].....
Document: Viral RNA was extracted from nasopharyngeal and fecal specimens with a QIAamp Viral RNA Mini Kit (QIAGEN), and cDNA was synthesized using SuperScript TM III reverse transcriptase (Invitrogen) with a random hexamer, as described previously [21] . The generated cDNA was subjected to PCR using the Expand High Fidelity PLUS PCR System (Roche) with primer sets specific to viruses, such as human coronaviruses [38] , WU polyomavirus [24] , and PMMV [31] .
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