Author: Nakamura, Shota; Yang, Cheng-Song; Sakon, Naomi; Ueda, Mayo; Tougan, Takahiro; Yamashita, Akifumi; Goto, Naohisa; Takahashi, Kazuo; Yasunaga, Teruo; Ikuta, Kazuyoshi; Mizutani, Tetsuya; Okamoto, Yoshiko; Tagami, Michihira; Morita, Ryoji; Maeda, Norihiro; Kawai, Jun; Hayashizaki, Yoshihide; Nagai, Yoshiyuki; Horii, Toshihiro; Iida, Tetsuya; Nakaya, Takaaki
Title: Direct Metagenomic Detection of Viral Pathogens in Nasal and Fecal Specimens Using an Unbiased High-Throughput Sequencing Approach Document date: 2009_1_19
ID: 1j8z8lak_33
Snippet: Viral RNA was extracted from nasopharyngeal and fecal specimens with a QIAamp Viral RNA Mini Kit (QIAGEN), and cDNA was synthesized using SuperScript TM III reverse transcriptase (Invitrogen) with a random hexamer, as described previously [21] . The generated cDNA was subjected to PCR using the Expand High Fidelity PLUS PCR System (Roche) with primer sets specific to viruses, such as human coronaviruses [38] , WU polyomavirus [24] , and PMMV [31].....
Document: Viral RNA was extracted from nasopharyngeal and fecal specimens with a QIAamp Viral RNA Mini Kit (QIAGEN), and cDNA was synthesized using SuperScript TM III reverse transcriptase (Invitrogen) with a random hexamer, as described previously [21] . The generated cDNA was subjected to PCR using the Expand High Fidelity PLUS PCR System (Roche) with primer sets specific to viruses, such as human coronaviruses [38] , WU polyomavirus [24] , and PMMV [31] .
Search related documents:
Co phrase search for related documents- generate cdna and reverse transcriptase: 1, 2, 3, 4, 5, 6
- Invitrogen SuperScript TM III reverse transcriptase and reverse transcriptase: 1
- Invitrogen SuperScript TM III reverse transcriptase and SuperScript TM III reverse transcriptase: 1
- Mini kit and primer set: 1, 2, 3, 4
- Mini kit and QIAamp viral RNA Mini kit: 1, 2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17, 18, 19, 20, 21, 22, 23, 24, 25, 26, 27, 28, 29, 30, 31, 32, 33, 34, 35, 36, 37, 38, 39, 40, 41, 42, 43, 44, 45, 46, 47, 48, 49, 50, 51, 52, 53, 54, 55, 56, 57, 58, 59
- Mini kit and QIAGEN QIAamp viral RNA Mini kit: 1, 2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17, 18, 19, 20, 21, 22, 23, 24, 25, 26, 27, 28, 29, 30, 31, 32, 33, 34, 35, 36, 37, 38, 39, 40, 41, 42, 43, 44, 45, 46
- Mini kit and random hexamer: 1, 2, 3, 4
- Mini kit and reverse transcriptase: 1, 2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15
- previously describe and reverse transcriptase: 1
- primer set and QIAamp viral RNA Mini kit: 1
- primer set and QIAGEN QIAamp viral RNA Mini kit: 1
- primer set and reverse transcriptase: 1, 2, 3, 4, 5, 6, 7, 8
- primer set and virus specific primer set: 1, 2
- QIAamp viral RNA Mini kit and QIAGEN QIAamp viral RNA Mini kit: 1, 2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17, 18, 19, 20, 21, 22, 23, 24, 25, 26, 27, 28, 29, 30, 31, 32, 33, 34, 35, 36, 37, 38, 39, 40, 41, 42, 43, 44, 45, 46
- QIAamp viral RNA Mini kit and random hexamer: 1
- QIAamp viral RNA Mini kit and reverse transcriptase: 1, 2, 3, 4
- QIAGEN QIAamp viral RNA Mini kit and random hexamer: 1
- QIAGEN QIAamp viral RNA Mini kit and reverse transcriptase: 1, 2, 3
- random hexamer and reverse transcriptase: 1, 2, 3, 4, 5, 6, 7, 8
Co phrase search for related documents, hyperlinks ordered by date