Selected article for: "PCR amplification and template dna"
Author: Nakamura, Shota; Yang, Cheng-Song; Sakon, Naomi; Ueda, Mayo; Tougan, Takahiro; Yamashita, Akifumi; Goto, Naohisa; Takahashi, Kazuo; Yasunaga, Teruo; Ikuta, Kazuyoshi; Mizutani, Tetsuya; Okamoto, Yoshiko; Tagami, Michihira; Morita, Ryoji; Maeda, Norihiro; Kawai, Jun; Hayashizaki, Yoshihide; Nagai, Yoshiyuki; Horii, Toshihiro; Iida, Tetsuya; Nakaya, Takaaki
Title: Direct Metagenomic Detection of Viral Pathogens in Nasal and Fecal Specimens Using an Unbiased High-Throughput Sequencing Approach
  • Document date: 2009_1_19
    • ID: 1j8z8lak_31
    Snippet: Total RNA was extracted from specimens with TRI-LS (Sigma-Aldrich), and was reverse-transcribed with the Transplex whole transcriptome amplification (WTA) kit (Sigma-Aldrich) [21] using a quasi-random primer, according to the manufacturer's protocol. PCR amplification for the preparation of template DNA for pyrosequencing was carried out by AmpliTaq Gold DNA Polymerase LD (Applied Biosystems) [21] . Norovirus-specific PCR was performed as describ.....
    Document: Total RNA was extracted from specimens with TRI-LS (Sigma-Aldrich), and was reverse-transcribed with the Transplex whole transcriptome amplification (WTA) kit (Sigma-Aldrich) [21] using a quasi-random primer, according to the manufacturer's protocol. PCR amplification for the preparation of template DNA for pyrosequencing was carried out by AmpliTaq Gold DNA Polymerase LD (Applied Biosystems) [21] . Norovirus-specific PCR was performed as described above [26] , and Flu-specific PCR was performed using the FluA M gene-specific primer set (M30F: 59-TTCTAACCGAGGTCGAAACG-39 and M264R2: 59-ACAAAGCGTCTACGCTGCAG-39).

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