Author: Ping Liu; Jing-Zhe Jiang; Xiu-Feng Wan; Yan Hua; Xiaohu Wang; Fanghui Hou; Jing Chen; Jiejian Zou; Jinping Chen
Title: Are pangolins the intermediate host of the 2019 novel coronavirus (2019-nCoV) ? Document date: 2020_2_20
ID: 2inlyd0t_30
Snippet: Through viral metagenomics analyses of lower respiratory tract samples from these pangolins, we detected coronavirus in three individual animals [11] . Two of these animals were from the first batch of Malayan pangolins intercepted by Meizhou, Yangjiang, and Jiangmen customs, and the third one was from the second batch in a freight being transported from Qingyuan to Heyuan. The RNA samples from these three individuals were subjected to deep seque.....
Document: Through viral metagenomics analyses of lower respiratory tract samples from these pangolins, we detected coronavirus in three individual animals [11] . Two of these animals were from the first batch of Malayan pangolins intercepted by Meizhou, Yangjiang, and Jiangmen customs, and the third one was from the second batch in a freight being transported from Qingyuan to Heyuan. The RNA samples from these three individuals were subjected to deep sequencing. To determine the read abundance of coronaviruses in each sample, we mapped clean reads without ribosomes and host sequences to an in-house virus reference data separated from the GenBank non-redundant nucleotide database.
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