Author: Nakamura, Shota; Yang, Cheng-Song; Sakon, Naomi; Ueda, Mayo; Tougan, Takahiro; Yamashita, Akifumi; Goto, Naohisa; Takahashi, Kazuo; Yasunaga, Teruo; Ikuta, Kazuyoshi; Mizutani, Tetsuya; Okamoto, Yoshiko; Tagami, Michihira; Morita, Ryoji; Maeda, Norihiro; Kawai, Jun; Hayashizaki, Yoshihide; Nagai, Yoshiyuki; Horii, Toshihiro; Iida, Tetsuya; Nakaya, Takaaki
Title: Direct Metagenomic Detection of Viral Pathogens in Nasal and Fecal Specimens Using an Unbiased High-Throughput Sequencing Approach Document date: 2009_1_19
ID: 1j8z8lak_11
Snippet: The Flu sequence was detected from all three samples in 21,858-30,958 (average 25,978) reads, as shown in Table 2 . A partial genome was covered in these samples, and the cover rate ranged 8.1-58.3% (Table 2 ). One major reason for the partial coverage might be the large amount of host-derived sequences (90.0-94.6%; Table 2 and Figure 2 ), which were due to our direct RNA isolation from nasopharyngeal aspirates without the elimination of host or .....
Document: The Flu sequence was detected from all three samples in 21,858-30,958 (average 25,978) reads, as shown in Table 2 . A partial genome was covered in these samples, and the cover rate ranged 8.1-58.3% (Table 2 ). One major reason for the partial coverage might be the large amount of host-derived sequences (90.0-94.6%; Table 2 and Figure 2 ), which were due to our direct RNA isolation from nasopharyngeal aspirates without the elimination of host or bacterial cells. However, 20-460 reads were Flu-derived, which was sufficient for subtype identification (H1N1 in sample #F2 and H3N2 in samples #F1 and #F3) from these sequences (Table S1 ).
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