Selected article for: "genome analysis and RNA preparation"
Author: Nakamura, Shota; Yang, Cheng-Song; Sakon, Naomi; Ueda, Mayo; Tougan, Takahiro; Yamashita, Akifumi; Goto, Naohisa; Takahashi, Kazuo; Yasunaga, Teruo; Ikuta, Kazuyoshi; Mizutani, Tetsuya; Okamoto, Yoshiko; Tagami, Michihira; Morita, Ryoji; Maeda, Norihiro; Kawai, Jun; Hayashizaki, Yoshihide; Nagai, Yoshiyuki; Horii, Toshihiro; Iida, Tetsuya; Nakaya, Takaaki
Title: Direct Metagenomic Detection of Viral Pathogens in Nasal and Fecal Specimens Using an Unbiased High-Throughput Sequencing Approach
  • Document date: 2009_1_19
    • ID: 1j8z8lak_21
    Snippet: In this study, we demonstrated the potential of the 454 parallel sequencing platform to identify pathogenic viruses from clinical specimens. We chose random RT-PCR for template cDNA preparation, because of the low levels of isolated RNA from the specimens. Flu and norovirus were detected from all three nasopharyngeal aspirates and five stool specimens, respectively, consistent with other diagnostic methods, including RT-PCR. In addition to these .....
    Document: In this study, we demonstrated the potential of the 454 parallel sequencing platform to identify pathogenic viruses from clinical specimens. We chose random RT-PCR for template cDNA preparation, because of the low levels of isolated RNA from the specimens. Flu and norovirus were detected from all three nasopharyngeal aspirates and five stool specimens, respectively, consistent with other diagnostic methods, including RT-PCR. In addition to these viruses, possible pathogenic viruses, such as WUV and HCoV-HKU1, were also detected in the nasopharyngeal aspirates and fecal samples, respectively, suggesting that this system (Figure 4) is useful for novel virus identification as well as for viral genome analysis.

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