Author: Qing, Enya; Hantak, Michael; Perlman, Stanley; Gallagher, Tom
Title: Distinct Roles for Sialoside and Protein Receptors in Coronavirus Infection Document date: 2020_2_11
ID: 1mowsbjy_34
Snippet: At 6 h posttransfection, effector and target cells were rinsed with PBS, lifted with 0.05% trypsin, and mixed at 1:1 ratios. The cocultures were incubated for 2 to 18 h at 37°C. Fused cells were visualized microscopically as green fluorescent protein-positive (GFP ϩ ) cells, and extents of cell-cell fusion were quantified as Rluc activities present in PLB cell lysates. Samples were chilled and maintained at 4°C during Rluc quantifications to e.....
Document: At 6 h posttransfection, effector and target cells were rinsed with PBS, lifted with 0.05% trypsin, and mixed at 1:1 ratios. The cocultures were incubated for 2 to 18 h at 37°C. Fused cells were visualized microscopically as green fluorescent protein-positive (GFP ϩ ) cells, and extents of cell-cell fusion were quantified as Rluc activities present in PLB cell lysates. Samples were chilled and maintained at 4°C during Rluc quantifications to eliminate DSP postlysis complementation. In time course experiments, EnduRen substrate (Promega E6482) was used in place of coelenterazine.
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