Author: Elsie Yekwa; Chutima Aphibanthammakit; Xavier Carnec; Bruno Coutard; Caroline Picard; Bruno Canard; Sylvain Baize; François Ferron
Title: Arenaviridae exoribonuclease presents genomic RNA edition capacity Document date: 2019_2_8
ID: fvp45ho6_7
Snippet: The PCR plates were sealed with optical sealing tape (Bio-rad) and incubated in the PCR machine for 2 minutes at 20°C followed by 0,2°C increments to a final temperature of 95°C. Thermal The copyright holder for this preprint (which was not peer-reviewed) is the . https://doi.org/10.1101/541698 doi: bioRxiv preprint intensities were measured at 490 nm excitation and 530 nm emission wavelengths. The unfolding of proteins was monitored by follow.....
Document: The PCR plates were sealed with optical sealing tape (Bio-rad) and incubated in the PCR machine for 2 minutes at 20°C followed by 0,2°C increments to a final temperature of 95°C. Thermal The copyright holder for this preprint (which was not peer-reviewed) is the . https://doi.org/10.1101/541698 doi: bioRxiv preprint intensities were measured at 490 nm excitation and 530 nm emission wavelengths. The unfolding of proteins was monitored by following the increase in fluorescence of the probe as it binds to exposed hydrophobic regions of the denatured protein. The Tm was then calculated as the mid-log of the transition phase of the florescence curve using the Boltzmann equation. All measurements were performed in triplicates.
Search related documents:
Co phrase search for related documents- denatured protein and nm emission wavelength: 1
- denatured protein and protein unfolding: 1
- emission wavelength and nm emission wavelength: 1, 2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17
- emission wavelength and nm excitation: 1, 2, 3, 4, 5, 6, 7, 8, 9, 10, 11
Co phrase search for related documents, hyperlinks ordered by date