Artesunate interacts with Vitamin D receptor to reverse mouse model of sepsis-induced immunosuppression via enhancing autophagy

Shenglan Shang; Jiaqi Wu; Xiaoli Li; Xin Liu; Pan Li; Chunli Zheng; Yonghua Wang; Songqing Liu; Jiang Zheng; Hong Zhou

. CC-BY-NC-ND 4.0 International license author/funder. It is made available under a The copyright holder for this preprint (which was not peer-reviewed) is the . https: //doi.org/10.1101 //doi.org/10. /2020 13 Mouse peritoneal macrophages (PMs) were obtained from BALB/c mice. Each mouse was intraperitoneal injected with 3 mL of 3% thioglycolate (Sigma-Aldrich) on day 1 and sacrificed using isoflurane on Day 3. After intraperitoneal injection of 5 mL DMEM regular medium, the peritoneal cells were collected into cell culture dishes. Two hours later, the floating cells were removed by washing the cells with phosphate-buffered saline (PBS). The attached cells were considered to be PMs (purity is about 90%) and were subjected to further experiments. The primary mouse macrophages or cell lines were maintained in regular medium at 37 °C in a humidified 5% CO 2 atmosphere. All the reagents and utensils used in the experiment were LPS-free.