Selected article for: "cell death and flow cytometry"
Author: Xing, Yang; Liqi, Zhu; Jian, Lin; Qinghua, Yu; Qian, Yang
Title: Doxycycline Induces Mitophagy and Suppresses Production of Interferon-ß in IPEC-J2 Cells
  • Document date: 2017_2_1
    • ID: 1ljye9pj_15
    Snippet: Apoptotic cell death was measured by Annexin V/propidium iodide (PI) staining assay (Miltenyi Biotec, Shanghai, China) according to manufacturer's instructions. In a word, cells were harvested and washed once with PBS, then resuspended in 100 µl binding buffer followed by incubation with 10 µl Annexin V per test for 20 min. Cells was washed again, suspended in 500 µl of binding buffer and 5 µl PI per test was added and immediately analyzed by.....
    Document: Apoptotic cell death was measured by Annexin V/propidium iodide (PI) staining assay (Miltenyi Biotec, Shanghai, China) according to manufacturer's instructions. In a word, cells were harvested and washed once with PBS, then resuspended in 100 µl binding buffer followed by incubation with 10 µl Annexin V per test for 20 min. Cells was washed again, suspended in 500 µl of binding buffer and 5 µl PI per test was added and immediately analyzed by flow cytometry (FACS). All data were analyzed using FlowJo software (Version 7.6.5, Tree Star Inc. Ashland, Oregon). All of the experiments were performed in three independent experiments. The relative intensity (%) was expressed as a percentage relative to the untreated control cells.

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