Author: Chernobrovkin, Alexey L.; Zubarev, Roman A.
Title: Detection of Viral Proteins in Human Cells Lines by Xeno-Proteomics: Elimination of the Last Valid Excuse for Not Testing Every Cellular Proteome Dataset for Viral Proteins Document date: 2014_3_11
ID: 0hrwqho8_4
Snippet: In contrast to contamination by microbes and mycoplasma, which can be relatively easily detected, viral contamination presents a serious threat because of the difficulty to detect some viruses and the lack of effective methods of curing infected cell cultures [13] . Cell banks commonly test cell lines for several widespread viral infections by using PCR or RT-PCR. However, even certified cell lines can still be contaminated by viruses that do not.....
Document: In contrast to contamination by microbes and mycoplasma, which can be relatively easily detected, viral contamination presents a serious threat because of the difficulty to detect some viruses and the lack of effective methods of curing infected cell cultures [13] . Cell banks commonly test cell lines for several widespread viral infections by using PCR or RT-PCR. However, even certified cell lines can still be contaminated by viruses that do not lead to a cytopathic effect. At DSMZ, only tests detecting human pathogen viruses have been performed for human cell lines [13] .
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