Selected article for: "fidelity polymerase and high fidelity polymerase"
Author: Van Nguyen, Dung; Van Nguyen, Cuong; Bonsall, David; Ngo, Tue Tri; Carrique-Mas, Juan; Pham, Anh Hong; Bryant, Juliet E.; Thwaites, Guy; Baker, Stephen; Woolhouse, Mark; Simmonds, Peter
Title: Detection and Characterization of Homologues of Human Hepatitis Viruses and Pegiviruses in Rodents and Bats in Vietnam
  • Document date: 2018_2_28
    • ID: 0jtfc271_18
    Snippet: For bat HBV, primers were designed using sequences available from Genbank and the obtained sequences from screening. These primers amplified amplicons, with overlapping regions as presented in Table S4 . All of these nested or hemi-nested PCR protocols used SuperScript III One-Step RT-PCR System with Platinum Taq DNA polymerase (Invitrogen, Paisley, UK) for RNA viruses or Q5 High-Fidelity DNA Polymerase (New England Biolabs, Hitchin, UK) for HBV .....
    Document: For bat HBV, primers were designed using sequences available from Genbank and the obtained sequences from screening. These primers amplified amplicons, with overlapping regions as presented in Table S4 . All of these nested or hemi-nested PCR protocols used SuperScript III One-Step RT-PCR System with Platinum Taq DNA polymerase (Invitrogen, Paisley, UK) for RNA viruses or Q5 High-Fidelity DNA Polymerase (New England Biolabs, Hitchin, UK) for HBV in the first round PCR, according to instructions from the manufacturers. Q5 High-Fidelity DNA Polymerase was also used in the second round PCR.

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