Selected article for: "copy number and linear regression"
Author: Moore, Matthew D.; Jaykus, Lee-Ann
Title: Development of a Recombinase Polymerase Amplification Assay for Detection of Epidemic Human Noroviruses
  • Document date: 2017_1_9
    • ID: 03a6hq3t_2
    Snippet: Evaluation of RT-RPA performance with GII.4 New Orleans outbreak samples. RT-RPA performance was tested using serially diluted stool samples obtained from 12 different individuals during 10 different GII. 4 New Orleans outbreaks that occurred in 2012. Two sample preparation methods were analyzed for each of the samples-conventional RNA purification and direct detection after boiling of patient stool ( Table 2) . RT-RPA applied to serially-diluted.....
    Document: Evaluation of RT-RPA performance with GII.4 New Orleans outbreak samples. RT-RPA performance was tested using serially diluted stool samples obtained from 12 different individuals during 10 different GII. 4 New Orleans outbreaks that occurred in 2012. Two sample preparation methods were analyzed for each of the samples-conventional RNA purification and direct detection after boiling of patient stool ( Table 2) . RT-RPA applied to serially-diluted purified RNA samples produced signal for a wide range of virus input levels, was highly repeatable, and identified virus in all patient samples. RNA copy number over a range of 0.2 log 10 genomic copies (LGC) to 9. 4 LGC per reaction produced positive signal fluorescence within 6.1 min to 21.5 min. Overall for purified RNA, the input LGC of RNA as determined by RT-qPCR was inversely proportional to the time to signal of the RT-RPA when analyzed by linear regression ( Figure S2 ).

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