Author: Qing, Enya; Hantak, Michael; Perlman, Stanley; Gallagher, Tom
Title: Distinct Roles for Sialoside and Protein Receptors in Coronavirus Infection Document date: 2020_2_11
ID: 1mowsbjy_37
Snippet: Western blotting. Proteins in sodium dodecyl sulfate (SDS) solubilizer (0.0625 M Tris·HCl [pH 6.8], 10% glycerol, 0.01% bromophenol blue, 2% [wt/vol] SDS, with 2% 2-mercaptoethanol] were heated at 95°C for 5 min, electrophoresed through 8% (wt/vol) polyacrylamide-SDS gels, transferred to nitrocellulose membranes (Bio-Rad), and incubated with mouse monoclonal anti-MHV-HR2 10G (obtained from Fumihiro Taguchi, Nippon Veterinary and Life Sciences, .....
Document: Western blotting. Proteins in sodium dodecyl sulfate (SDS) solubilizer (0.0625 M Tris·HCl [pH 6.8], 10% glycerol, 0.01% bromophenol blue, 2% [wt/vol] SDS, with 2% 2-mercaptoethanol] were heated at 95°C for 5 min, electrophoresed through 8% (wt/vol) polyacrylamide-SDS gels, transferred to nitrocellulose membranes (Bio-Rad), and incubated with mouse monoclonal anti-MHV-HR2 10G (obtained from Fumihiro Taguchi, Nippon Veterinary and Life Sciences, Tokyo, Japan), mouse anti-C9 (EMD Millipore), mouse monoclonal anti-MHV HE, clone 5A11, or goat anti-human IgG (sc-2453; Santa Cruz Biotechnologies). After incubation with appropriate horseradish peroxidase (HRP)-tagged secondary antibodies and chemiluminescent substrate (Thermo Fisher), the blots were imaged and processed with a FluorChem E (Protein Simple).
Search related documents:
Co phrase search for related documents- appropriate horseradish peroxidase and horseradish peroxidase: 1, 2, 3, 4
- appropriate horseradish peroxidase and polyacrylamide SDS gel: 1
- appropriate horseradish peroxidase and secondary antibody: 1, 2
- appropriate horseradish peroxidase and western blotting: 1
- Bio Rad membrane and horseradish peroxidase: 1, 2, 3
- Bio Rad membrane and polyacrylamide SDS gel: 1
- Bio Rad membrane and secondary antibody: 1, 2, 3, 4, 5
- Bio Rad membrane and western blotting: 1, 2, 3
- Bio Rad membrane nitrocellulose and horseradish peroxidase: 1, 2
- Bio Rad membrane nitrocellulose and secondary antibody: 1, 2, 3, 4
- Bio Rad membrane nitrocellulose and western blotting: 1
- chemiluminescent substrate and horseradish peroxidase: 1, 2, 3, 4, 5, 6, 7
- chemiluminescent substrate and polyacrylamide SDS gel: 1
- chemiluminescent substrate and secondary antibody: 1, 2
- chemiluminescent substrate and western blotting: 1, 2, 3, 4, 5
- horseradish peroxidase and polyacrylamide SDS gel: 1, 2, 3, 4, 5, 6, 7, 8, 9
- horseradish peroxidase and secondary antibody: 1, 2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17, 18, 19, 20, 21, 22, 23, 24, 25
- horseradish peroxidase and sodium protein: 1, 2
- horseradish peroxidase and western blotting: 1, 2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17, 18
Co phrase search for related documents, hyperlinks ordered by date