Selected article for: "peroxidase substrate and western blotting"
Author: Qing, Enya; Hantak, Michael; Perlman, Stanley; Gallagher, Tom
Title: Distinct Roles for Sialoside and Protein Receptors in Coronavirus Infection
  • Document date: 2020_2_11
    • ID: 1mowsbjy_37
    Snippet: Western blotting. Proteins in sodium dodecyl sulfate (SDS) solubilizer (0.0625 M Tris·HCl [pH 6.8], 10% glycerol, 0.01% bromophenol blue, 2% [wt/vol] SDS, with 2% 2-mercaptoethanol] were heated at 95°C for 5 min, electrophoresed through 8% (wt/vol) polyacrylamide-SDS gels, transferred to nitrocellulose membranes (Bio-Rad), and incubated with mouse monoclonal anti-MHV-HR2 10G (obtained from Fumihiro Taguchi, Nippon Veterinary and Life Sciences, .....
    Document: Western blotting. Proteins in sodium dodecyl sulfate (SDS) solubilizer (0.0625 M Tris·HCl [pH 6.8], 10% glycerol, 0.01% bromophenol blue, 2% [wt/vol] SDS, with 2% 2-mercaptoethanol] were heated at 95°C for 5 min, electrophoresed through 8% (wt/vol) polyacrylamide-SDS gels, transferred to nitrocellulose membranes (Bio-Rad), and incubated with mouse monoclonal anti-MHV-HR2 10G (obtained from Fumihiro Taguchi, Nippon Veterinary and Life Sciences, Tokyo, Japan), mouse anti-C9 (EMD Millipore), mouse monoclonal anti-MHV HE, clone 5A11, or goat anti-human IgG (sc-2453; Santa Cruz Biotechnologies). After incubation with appropriate horseradish peroxidase (HRP)-tagged secondary antibodies and chemiluminescent substrate (Thermo Fisher), the blots were imaged and processed with a FluorChem E (Protein Simple).

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