Author: Xing, Yang; Liqi, Zhu; Jian, Lin; Qinghua, Yu; Qian, Yang
Title: Doxycycline Induces Mitophagy and Suppresses Production of Interferon-ß in IPEC-J2 Cells Document date: 2017_2_1
ID: 1ljye9pj_12
Snippet: The fluorescent probe 6-carboxy-2 ′ , 7 ′dichorodihydrofluorescein diacetate (DCFH-DA) and mitochondrial superoxide indicator MitoSox Red (Life Technologies) were used to measure the intracellular production of ROS or mitochondrial ROS (mitoROS), respectively. After 24 h treatment with DOX, cells were incubated with 10 µM DCFH-DA or 5 µM MitoSox serum-free medium for 10 min at 37 • C. Afterwards, cells were harvested and resuspended in 50.....
Document: The fluorescent probe 6-carboxy-2 ′ , 7 ′dichorodihydrofluorescein diacetate (DCFH-DA) and mitochondrial superoxide indicator MitoSox Red (Life Technologies) were used to measure the intracellular production of ROS or mitochondrial ROS (mitoROS), respectively. After 24 h treatment with DOX, cells were incubated with 10 µM DCFH-DA or 5 µM MitoSox serum-free medium for 10 min at 37 • C. Afterwards, cells were harvested and resuspended in 500 µl of PBS, and DCF and MitoSox Red fluorescence were measured by FACS.
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