Author: Thomas, Joseph T.; Chen, Qi; Gauger, Phillip C.; Giménez-Lirola, Luis G.; Sinha, Avanti; Harmon, Karen M.; Madson, Darin M.; Burrough, Eric R.; Magstadt, Drew R.; Salzbrenner, Holly M.; Welch, Michael W.; Yoon, Kyoung-Jin; Zimmerman, Jeffrey J.; Zhang, Jianqiang
Title: Effect of Porcine Epidemic Diarrhea Virus Infectious Doses on Infection Outcomes in Naïve Conventional Neonatal and Weaned Pigs Document date: 2015_10_6
ID: 1kjk404o_3
Snippet: Real-time RT-PCR (rRT-PCR) has been widely used for PEDV detection and diagnosis. However, correlations of PEDV infectious titers to the rRT-PCR cycle threshold (Ct) values have not been described. Virus isolation has generally been used to assess if a live virus is present in samples. But for PEDV, the success rate of virus isolation in cell culture has been quite low [22] . Currently, swine bioassay remains the most reliable means to determine .....
Document: Real-time RT-PCR (rRT-PCR) has been widely used for PEDV detection and diagnosis. However, correlations of PEDV infectious titers to the rRT-PCR cycle threshold (Ct) values have not been described. Virus isolation has generally been used to assess if a live virus is present in samples. But for PEDV, the success rate of virus isolation in cell culture has been quite low [22] . Currently, swine bioassay remains the most reliable means to determine if infectious PEDV is present in a clinical specimen or if "X" treatment will inactivate the virus. However, there remains inconsistency in selecting swine models for bioassay.
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