Author: Kang, Hee; Park, Sung-Hyun; Yun, Jeong-Moon; Nam, Tae-Gyu; Kim, Young-Eun; Kim, Dae-Ok; Kim, Youn Jung
Title: Effect of cinnamon water extract on monocyte-to-macrophage differentiation and scavenger receptor activity Document date: 2014_3_7
ID: 0bqhbm9p_22
Snippet: Cells were incubated with Alexa 488 -acetyl LDL (2.5 μg/ml) (Molecular Probes, USA) for 16 h in PMA-stimulated THP-1 cells or for 2 h in mouse peritoneal macrophages. Fucoidan (75 μg/ml) (Sigma) or U0126 (20 μM) (Sigma) was incubated 24 h before the addition of Alexa 488 -acetyl LDL. Cells were washed and harvested with PBS and then analyzed on a FACSAria (BD Biosciences) with the use of FACSDiva software. For every sample, the mean fluorescen.....
Document: Cells were incubated with Alexa 488 -acetyl LDL (2.5 μg/ml) (Molecular Probes, USA) for 16 h in PMA-stimulated THP-1 cells or for 2 h in mouse peritoneal macrophages. Fucoidan (75 μg/ml) (Sigma) or U0126 (20 μM) (Sigma) was incubated 24 h before the addition of Alexa 488 -acetyl LDL. Cells were washed and harvested with PBS and then analyzed on a FACSAria (BD Biosciences) with the use of FACSDiva software. For every sample, the mean fluorescence intensity of cells was determined.
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