Selected article for: "antigen detection and monoclonal antibody"
Author: Thomas, Joseph T.; Chen, Qi; Gauger, Phillip C.; Giménez-Lirola, Luis G.; Sinha, Avanti; Harmon, Karen M.; Madson, Darin M.; Burrough, Eric R.; Magstadt, Drew R.; Salzbrenner, Holly M.; Welch, Michael W.; Yoon, Kyoung-Jin; Zimmerman, Jeffrey J.; Zhang, Jianqiang
Title: Effect of Porcine Epidemic Diarrhea Virus Infectious Doses on Infection Outcomes in Naïve Conventional Neonatal and Weaned Pigs
  • Document date: 2015_10_6
    • ID: 1kjk404o_25
    Snippet: Serial sections of ileums were evaluated for PEDV antigen by immunohistochemistry (IHC) using a PEDV-specific monoclonal antibody (BioNote, Hwaseong-si, Gyeonggi-do, Korea) as previously described [24] . The IHC antigen detection was semi-quantitatively scored as previously described [26] with the following criteria: 0 = no staining; 1 = approximately 1-10% enterocytes with positive staining; 2 = approximately 10%-25% enterocytes with positive st.....
    Document: Serial sections of ileums were evaluated for PEDV antigen by immunohistochemistry (IHC) using a PEDV-specific monoclonal antibody (BioNote, Hwaseong-si, Gyeonggi-do, Korea) as previously described [24] . The IHC antigen detection was semi-quantitatively scored as previously described [26] with the following criteria: 0 = no staining; 1 = approximately 1-10% enterocytes with positive staining; 2 = approximately 10%-25% enterocytes with positive staining; 3 = approximately 25%-50% enterocytes with positive staining; 4 = approximately 50%-100% enterocytes with positive staining.

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