Selected article for: "cell binding and host cell"
Author: Qing, Enya; Hantak, Michael; Perlman, Stanley; Gallagher, Tom
Title: Distinct Roles for Sialoside and Protein Receptors in Coronavirus Infection
  • Document date: 2020_2_11
    • ID: 1mowsbjy_2
    Snippet: To validate the presumed JHM virus spike-host cell sialic acid interaction, we developed an MHV-based virus-like particle (VLP) system, in which VLPs contained reporter proteins that can be tracked during virus-cell binding and entry (Fig. 1B ). In this system, VLPs were produced from 293T cells cotransfected with MHV structural genes S, E, M, and N (38) (39) (40) . The N genes were modified by in-frame fusion with dual-split protein (DSP) encodi.....
    Document: To validate the presumed JHM virus spike-host cell sialic acid interaction, we developed an MHV-based virus-like particle (VLP) system, in which VLPs contained reporter proteins that can be tracked during virus-cell binding and entry (Fig. 1B ). In this system, VLPs were produced from 293T cells cotransfected with MHV structural genes S, E, M, and N (38) (39) (40) . The N genes were modified by in-frame fusion with dual-split protein (DSP) encoding sequences, the two of which complement to form Renilla luciferase (Rluc) and green fluorescent protein (GFP) reporters. This allowed for the release of VLPs that were intrinsically Rluc and GFP positive.

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