Author: Qing, Enya; Hantak, Michael; Perlman, Stanley; Gallagher, Tom
Title: Distinct Roles for Sialoside and Protein Receptors in Coronavirus Infection Document date: 2020_2_11
ID: 1mowsbjy_31
Snippet: VLP entry assay. VLPs used for entry assays were prepared by cotransfection with plasmids encoding CoV S, E (envelope), M (matrix), and DSP 8-11 -N. The resulting VLPs contained DSP fragments in their interiors. To quantify VLP-associated DSP levels, VLPs were mixed with excess DSP 1-7 , in passive lysis buffer, for 30 min at 37°C, to allow for DSP complementation. The excess DSP 1-7 was obtained from HEK293 cells overexpressing these fragments......
Document: VLP entry assay. VLPs used for entry assays were prepared by cotransfection with plasmids encoding CoV S, E (envelope), M (matrix), and DSP 8-11 -N. The resulting VLPs contained DSP fragments in their interiors. To quantify VLP-associated DSP levels, VLPs were mixed with excess DSP 1-7 , in passive lysis buffer, for 30 min at 37°C, to allow for DSP complementation. The excess DSP 1-7 was obtained from HEK293 cells overexpressing these fragments. After postlysis complementation, samples were introduced into opaque microplate wells and luminescence readings were used to infer DSP [8] [9] [10] [11] levels.
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