Author: Qing, Enya; Hantak, Michael; Perlman, Stanley; Gallagher, Tom
Title: Distinct Roles for Sialoside and Protein Receptors in Coronavirus Infection Document date: 2020_2_11
ID: 1mowsbjy_32
Snippet: VLPs in FBS-free DMEM were inoculated onto target cells that were transfected 2 days earlier with pDSP [1] [2] [3] [4] [5] [6] [7] . Indicated experiments included cotransfection of target cells with pDSP 1-7 and pCAGGS-TMPRSS2 FLAG (44) . For VLP inoculations, input multiplicities were normalized to VLP DSP 8-11 levels. After 6 h at 37°C, the cells were rinsed three times with PBS and dissolved in passive lysis buffer, and Rluc activities were .....
Document: VLPs in FBS-free DMEM were inoculated onto target cells that were transfected 2 days earlier with pDSP [1] [2] [3] [4] [5] [6] [7] . Indicated experiments included cotransfection of target cells with pDSP 1-7 and pCAGGS-TMPRSS2 FLAG (44) . For VLP inoculations, input multiplicities were normalized to VLP DSP 8-11 levels. After 6 h at 37°C, the cells were rinsed three times with PBS and dissolved in passive lysis buffer, and Rluc activities were quantified.
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