Author: Meng, Fandan; Wu, Nai-Huei; Seitz, Maren; Herrler, Georg; Valentin-Weigand, Peter
Title: Efficient suilysin-mediated invasion and apoptosis in porcine respiratory epithelial cells after streptococcal infection under air-liquid interface conditions Document date: 2016_5_27
ID: 0jsc81sy_43
Snippet: Immunofluorescence microscopy and field emission scanning electron microscopy (FESEM). The following primary antibodies were used in this study: Cy3-labeled monoclonal antibody against β -tubulin (1:400), mucin-5 AC antibody (1:250, Santa Cruz Biotechnology), polyclonal antibody against cleaved caspase-3 (1:400, Cell Signaling), occludin alexa fluor 488 conjugate antibody (1:200, invitrogen), polyclonal rabbit serum raised against suilysin (1:13.....
Document: Immunofluorescence microscopy and field emission scanning electron microscopy (FESEM). The following primary antibodies were used in this study: Cy3-labeled monoclonal antibody against β -tubulin (1:400), mucin-5 AC antibody (1:250, Santa Cruz Biotechnology), polyclonal antibody against cleaved caspase-3 (1:400, Cell Signaling), occludin alexa fluor 488 conjugate antibody (1:200, invitrogen), polyclonal rabbit serum raised against suilysin (1:1300) 35 , and a polyclonal rabbit serum raised against S. suis (1:200) 35 .
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