Author: Thomas, Joseph T.; Chen, Qi; Gauger, Phillip C.; Giménez-Lirola, Luis G.; Sinha, Avanti; Harmon, Karen M.; Madson, Darin M.; Burrough, Eric R.; Magstadt, Drew R.; Salzbrenner, Holly M.; Welch, Michael W.; Yoon, Kyoung-Jin; Zimmerman, Jeffrey J.; Zhang, Jianqiang
Title: Effect of Porcine Epidemic Diarrhea Virus Infectious Doses on Infection Outcomes in Naïve Conventional Neonatal and Weaned Pigs Document date: 2015_10_6
ID: 1kjk404o_36
Snippet: Each virus dilution was also tested in triplicate by a quantitative PEDV N gene-based rRT-PCR. The virus stock had an average Ct of 12.2 and the Ct values increased by roughly 3-4 for every 10-fold dilution until the 10 −7 dilution that had a Ct value of 37.62, beyond which all dilutions had Ct>45 (Table 1 ). This range of Ct values correlated to 2.1×10 10 genomic copies/ml in the virus stock through 689 genomic copies/ml in the 10 −7 diluti.....
Document: Each virus dilution was also tested in triplicate by a quantitative PEDV N gene-based rRT-PCR. The virus stock had an average Ct of 12.2 and the Ct values increased by roughly 3-4 for every 10-fold dilution until the 10 −7 dilution that had a Ct value of 37.62, beyond which all dilutions had Ct>45 (Table 1 ). This range of Ct values correlated to 2.1×10 10 genomic copies/ml in the virus stock through 689 genomic copies/ml in the 10 −7 dilution, and an undetected number of genomic copies/ml in all dilutions beyond that ( Table 1) .
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